# Analyze striatal 10x data by Seurat in R

**URL:** <https://community.brain-map.org/t/analyze-striatal-10x-data-by-seurat-in-r/2899>\
**Category:** Allen Brain Cell (ABC) Atlas\
**Created:** [January 11, 2024, 3:49am UTC](https://community.brain-map.org/t/analyze-striatal-10x-data-by-seurat-in-r/2899 "2024-01-11T03:49:55Z")\
**Posts on this page:** 2\
**Page:** 1

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**Author:** ![lingwu217](https://avatars.discourse-cdn.com/v4/letter/l/b4bc9f/32.png) [@lingwu217](https://community.brain-map.org/u/lingwu217)\
**Post date:** [January 11, 2024, 3:49am UTC](https://community.brain-map.org/t/analyze-striatal-10x-data-by-seurat-in-r/2899/1 "2024-01-11T03:49:55Z")

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Hi thanks for the incredible resource!  
I am a single cell beginner and was trying to find the 10x v3 data that’s disected from striatum and analyze in R by Seurat.  
Could you help to locate the files?  
Does the matrix files under this link :  
ttps://data.nemoarchive.org/biccn/grant/u19\_zeng/zeng/transcriptome/scell/10x\_v3/mouse/process ed/analysis/10X\_cells\_v3\_AIBS/  
contain all the v3 data including other brain regions?  
Could you suggest a good way to isolate striatum data and analyze in R？  
Is it plausible to use the h5ad file that’s uploaded in AWS?

Thanks a lot!  
Ling

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**Author:** ![ray.sanchez](https://yyz1.discourse-cdn.com/flex027/user_avatar/community.brain-map.org/ray.sanchez/32/679_2.png) [@ray.sanchez](https://community.brain-map.org/u/ray.sanchez)\
**Post date:** [January 16, 2024, 5:58pm UTC](https://community.brain-map.org/t/analyze-striatal-10x-data-by-seurat-in-r/2899/2 "2024-01-16T17:58:50Z")

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Hi Ling,

Thanks for your question! The whole mouse brain atlas dataset does indeed include cells obtained from the striatum. I’d recommend following [these tutorial notebooks](https://alleninstitute.github.io/abc_atlas_access/descriptions/WMB-10Xv3.html) for examples of how to subset the data to meet your specific needs. Hopefully this helps!

Thanks,  
Ray
