# Are raw intracellular recording potential traces compensated for offset potential?

**URL:** <https://community.brain-map.org/t/are-raw-intracellular-recording-potential-traces-compensated-for-offset-potential/3854>\
**Category:** Science\
**Tags:** analysis, allensdk\
**Created:** [November 15, 2024, 3:43pm UTC](https://community.brain-map.org/t/are-raw-intracellular-recording-potential-traces-compensated-for-offset-potential/3854 "2024-11-15T15:43:10Z")\
**Posts on this page:** 2\
**Page:** 1

<div class="post-metadata">

**Author:** ![Jballbe](https://yyz1.discourse-cdn.com/flex027/user_avatar/community.brain-map.org/jballbe/32/610_2.png) [@Jballbe](https://community.brain-map.org/u/Jballbe)\
**Post date:** [November 15, 2024, 3:43pm UTC](https://community.brain-map.org/t/are-raw-intracellular-recording-potential-traces-compensated-for-offset-potential/3854/1 "2024-11-15T15:43:10Z")

</div>

Hi,

I am analyzing the raw traces of intracellular recordings. I noticed that in the Gouwens et al 2019 paper, it is mentioned :

> Data were reported uncorrected for the measured –14 mV liquid junction potential between the electrode and bath solutions

Does that mean that the traces in the NWB files , notably for long square protocols, should be down shifted by 14mV? Or are the traces already compensated for the offset potential, and are ready to use? I notably checked the values form a GLIF 3 model, and the “EL\_reference” parameter matched the traces from a nwb file, so it would seem the traces are actually corrected ?

Thank you for your help,

Best

Julien

---

<div class="post-metadata">

**Author:** ![gouwens](https://avatars.discourse-cdn.com/v4/letter/g/bcef8e/32.png) [@gouwens](https://community.brain-map.org/u/gouwens)\
**Post date:** [November 20, 2024, 4:53pm UTC](https://community.brain-map.org/t/are-raw-intracellular-recording-potential-traces-compensated-for-offset-potential/3854/2 "2024-11-20T16:53:23Z")

</div>

They are not adjusted for the liquid junction potential. I believe the outputs from the models were translated afterwards to match the uncorrected traces (so that they would be comparable in the web app, etc.).
