# Data download request for the "Developing Mouse Brain" scRNA-seq reference (van Velthoven et al., Nature 2025)

**URL:** <https://community.brain-map.org/t/data-download-request-for-the-developing-mouse-brain-scrna-seq-reference-van-velthoven-et-al-nature-2025/4785>\
**Category:** Technical\
**Tags:** atlas-mouse-brain-developing\
**Created:** [November 29, 2025, 1:35am UTC](https://community.brain-map.org/t/data-download-request-for-the-developing-mouse-brain-scrna-seq-reference-van-velthoven-et-al-nature-2025/4785 "2025-11-29T01:35:09Z")\
**Posts on this page:** 3\
**Page:** 1

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**Author:** ![johnmuin](https://yyz1.discourse-cdn.com/flex027/user_avatar/community.brain-map.org/johnmuin/32/2450_2.png) [@johnmuin](https://community.brain-map.org/u/johnmuin)\
**Post date:** [November 29, 2025, 1:35am UTC](https://community.brain-map.org/t/data-download-request-for-the-developing-mouse-brain-scrna-seq-reference-van-velthoven-et-al-nature-2025/4785/1 "2025-11-29T01:35:09Z")

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Dear Allen Institute Team,

I am analyzing scRNA-seq data from the **E15.5 mouse telencephalon** and looking for the corresponding reference dataset for label transfer.

I am referring to the new publication:  
_ **“Transcriptomic and spatial organization of telencephalic GABAergic neurons”** _ (van Velthoven et al., Nature 2025).

I am particularly interested in the **4-level taxonomy** (Class / Subclass / Supertype / Cluster) for embryonic GABAergic lineages (e.g., MGE/LGE Immature, STR D1/D2) as described in this paper.

Could you please provide a direct link (e.g., to NeMO Archive) to download the **annotated single-cell expression matrix (.h5ad)** that corresponds to this specific developmental study?

I have found the adult (P56) datasets, but I am specifically looking for the **embryonic/developing** dataset to match my E15.5 samples.

Thank you very much!

John Muin

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**Author:** ![cvanv](https://avatars.discourse-cdn.com/v4/letter/c/ce73a5/32.png) [@cvanv](https://community.brain-map.org/u/cvanv)\
**Post date:** [December 1, 2025, 7:37pm UTC](https://community.brain-map.org/t/data-download-request-for-the-developing-mouse-brain-scrna-seq-reference-van-velthoven-et-al-nature-2025/4785/2 "2025-12-01T19:37:34Z")

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Hi John,

this is the NeMO link to the processed files. It contains both the P56 as well as the developmental data.

> **[NeMO Identifiers: nemo:dat-zoiw2n4](https://assets.nemoarchive.org/collection/nemo:dat-zoiw2n4)**

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<div class="post-metadata">

**Author:** ![johnmuin](https://yyz1.discourse-cdn.com/flex027/user_avatar/community.brain-map.org/johnmuin/32/2450_2.png) [@johnmuin](https://community.brain-map.org/u/johnmuin)\
**Post date:** [December 8, 2025, 3:16am UTC](https://community.brain-map.org/t/data-download-request-for-the-developing-mouse-brain-scrna-seq-reference-van-velthoven-et-al-nature-2025/4785/3 "2025-12-08T03:16:37Z")

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Thank you very much.