# Injection site coordinates

**URL:** <https://community.brain-map.org/t/injection-site-coordinates/1444>\
**Category:** Technical\
**Tags:** allensdk\
**Created:** [February 8, 2022, 10:35am UTC](https://community.brain-map.org/t/injection-site-coordinates/1444 "2022-02-08T10:35:17Z")\
**Posts on this page:** 2\
**Page:** 1

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**Author:** ![Margaux](https://avatars.discourse-cdn.com/v4/letter/m/9d8465/32.png) [@Margaux](https://community.brain-map.org/u/Margaux)\
**Post date:** [February 8, 2022, 10:35am UTC](https://community.brain-map.org/t/injection-site-coordinates/1444/1 "2022-02-08T10:35:17Z")

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Hello,

I’m in the mouse connectivity dataset and I have the precise coordinates of the injection site [8870 , 860 , 792]. I know that the injection is in the primary visual area but is it possible to precisely know with allensdk in which layer it happened ?

Thanks !  
Margaux

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<div class="post-metadata">

**Author:** ![lydian](https://yyz1.discourse-cdn.com/flex027/user_avatar/community.brain-map.org/lydian/32/174_2.png) [@lydian](https://community.brain-map.org/u/lydian)\
**Post date:** [February 16, 2022, 5:30pm UTC](https://community.brain-map.org/t/injection-site-coordinates/1444/2 "2022-02-16T17:30:55Z")

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Hi Margaux,

From the injection site coordinate [8870,860,792] you provided, I believe you are referring to experiment [263780729](http://connectivity.brain-map.org/projection/experiment/263780729).

From the experiment page you can see that the injection site is large and spans multiple cortical layers.  
 ![image](https://canada1.discourse-cdn.com/flex027/uploads/brainobservatory/original/1X/8b2cbe2c609bbfdf69e8d401b4f38511b8e91b85.jpeg)

The coordinates [8870,860,792] should be interpreted as point inside of a 0.22 mm^3 injection volume.

**While it is possible use AllenSDK (start with this [tutorial](https://allensdk.readthedocs.io/en/latest/_static/examples/nb/reference_space.html)) to figure out what cortical layer CCF coordinate [8870,860,762] lies in, it cannot be interpreted that the injection site is localized to that layer.**
