# Is there documentation for neuropil trace calculation? Visual Coding 2P

**URL:** <https://community.brain-map.org/t/is-there-documentation-for-neuropil-trace-calculation-visual-coding-2p/3585>\
**Category:** Science\
**Tags:** experiment-design, brain-observatory-visual-coding\
**Created:** [August 19, 2024, 6:29pm UTC](https://community.brain-map.org/t/is-there-documentation-for-neuropil-trace-calculation-visual-coding-2p/3585 "2024-08-19T18:29:48Z")\
**Posts on this page:** 3\
**Page:** 1

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**Author:** ![nas225](https://avatars.discourse-cdn.com/v4/letter/n/ba9def/32.png) [@nas225](https://community.brain-map.org/u/nas225)\
**Post date:** [August 19, 2024, 6:29pm UTC](https://community.brain-map.org/t/is-there-documentation-for-neuropil-trace-calculation-visual-coding-2p/3585/1 "2024-08-19T18:29:48Z")

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Hi everyone,

I’m working with the Visual Coding 2P data, and I’m interested in understanding what methods and calculations were used to derive the provided neuropil traces. So far, I haven’t been able to find any details in the technical whitepapers or in other documentation online. If anyone could point me towards relevant resources or share insights on this process, I would greatly appreciate it.

Thank you!

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**Author:** ![saskiad](https://yyz1.discourse-cdn.com/flex027/user_avatar/community.brain-map.org/saskiad/32/1695_2.png) [@saskiad](https://community.brain-map.org/u/saskiad)\
**Post date:** [August 31, 2024, 2:12am UTC](https://community.brain-map.org/t/is-there-documentation-for-neuropil-trace-calculation-visual-coding-2p/3585/2 "2024-08-31T02:12:33Z")

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This is documented in the Visual Coding Overview white paper that can be found here ([Documentation: Brain Observatory](https://community.brain-map.org/t/documentation-brain-observatory/3026)) as well as in the Methods section of our platform paper found here: [A large-scale standardized physiological survey reveals functional organization of the mouse visual cortex | Nature Neuroscience](https://www.nature.com/articles/s41593-019-0550-9)

Good luck!

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**Author:** ![nas225](https://avatars.discourse-cdn.com/v4/letter/n/ba9def/32.png) [@nas225](https://community.brain-map.org/u/nas225)\
**Post date:** [September 4, 2024, 12:25am UTC](https://community.brain-map.org/t/is-there-documentation-for-neuropil-trace-calculation-visual-coding-2p/3585/3 "2024-09-04T00:25:53Z")

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Thank you so much! Just to be absolutely certain: the neuropil corrected traces are obtained by get\_corrected\_fluorescence, and neuropil is obtained by get\_neuropil?