# Paper for RNA-Seq data

**URL:** <https://community.brain-map.org/t/paper-for-rna-seq-data/171>\
**Category:** Technical\
**Tags:** atlas-human-brain-adult, publication\
**Created:** [April 29, 2019, 10:50am UTC](https://community.brain-map.org/t/paper-for-rna-seq-data/171 "2019-04-29T10:50:56Z")\
**Posts on this page:** 1\
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**Author:** ![jeremyinseattle](https://yyz1.discourse-cdn.com/flex027/user_avatar/community.brain-map.org/jeremyinseattle/32/50_2.png) [@jeremyinseattle](https://community.brain-map.org/u/jeremyinseattle)\
**Post date:** [April 29, 2019, 5:05pm UTC](https://community.brain-map.org/t/paper-for-rna-seq-data/171/2 "2019-04-29T17:05:42Z")

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The RNA-seq data from these two human brains is described in [this publication](https://www.ncbi.nlm.nih.gov/pubmed/24564186). In this paper, we performed a calibration experiment where we ran RNA-seq (~250 samples) and microarray data on the same samples and assigned quality scores for microarray probes based on their correlation with RNA-seq gene expression. This also allowed us to define more accurate measures of absolute expression levels for microarray probes. We performed a similar calibration using ~40 RNA-seq samples for our NIH Blueprint NHP Atlas [in this publication](https://www.ncbi.nlm.nih.gov/pubmed/27409810).

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