# Quantification of gene expression by in situ hybridization: finding and using the raw values

**URL:** <https://community.brain-map.org/t/quantification-of-gene-expression-by-in-situ-hybridization-finding-and-using-the-raw-values/153>\
**Category:** Science\
**Tags:** in-situ-hybridization, annotation, api, analysis, how-to, developing\
**Created:** [April 19, 2019, 1:54am UTC](https://community.brain-map.org/t/quantification-of-gene-expression-by-in-situ-hybridization-finding-and-using-the-raw-values/153 "2019-04-19T01:54:28Z")\
**Posts on this page:** 1\
**Showing post:** 2

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**Author:** ![jeremyinseattle](https://yyz1.discourse-cdn.com/flex027/user_avatar/community.brain-map.org/jeremyinseattle/32/50_2.png) [@jeremyinseattle](https://community.brain-map.org/u/jeremyinseattle)\
**Post date:** [May 1, 2019, 12:20am UTC](https://community.brain-map.org/t/quantification-of-gene-expression-by-in-situ-hybridization-finding-and-using-the-raw-values/153/2 "2019-05-01T00:20:41Z")

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We have also received several questions about how to perform structure unionization and return the output for more than one gene at a time (along with associated gene symbols). The API call below shows an example for how to do this for the genes _Fam84b_, _Fam184a_, and _Rasa_ and return the results in json format. Several online json to csv conversion tools are available (via a Google search) if you are uncomfortable with this format.

[http://api.brain-map.org/api/v2/data/query.json?criteria=model::StructureUnionize,rma::criteria,section\_data\_set(genes[acronym$in'Fam84b','Fam184a','Rasa2']),rma::include,section\_data\_set(genes),rma::options[only$eq'genes.acronym,data\_sets.id'][start\_row$eq8000][num\_rows$eq2000]](http://api.brain-map.org/api/v2/data/query.json?criteria=model::StructureUnionize,rma::criteria,section_data_set(genes%5Bacronym%24in%27Fam84b%27,%27Fam184a%27,%27Rasa2%27%5D),rma::include,section_data_set(genes),rma::options%5Bonly%24eq%27genes.acronym,data_sets.id%27%5D%5Bstart_row%24eq8000%5D%5Bnum_rows%24eq2000%5D)

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