# Questions on house keeping genes:

**URL:** <https://community.brain-map.org/t/questions-on-house-keeping-genes/1726>\
**Category:** Science\
**Created:** [July 18, 2022, 4:23pm UTC](https://community.brain-map.org/t/questions-on-house-keeping-genes/1726 "2022-07-18T16:23:34Z")\
**Posts on this page:** 2\
**Page:** 1

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**Author:** ![cjohnson23](https://avatars.discourse-cdn.com/v4/letter/c/d2c977/32.png) [@cjohnson23](https://community.brain-map.org/u/cjohnson23)\
**Post date:** [July 18, 2022, 4:23pm UTC](https://community.brain-map.org/t/questions-on-house-keeping-genes/1726/1 "2022-07-18T16:23:34Z")

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What are good house keeping genes to use with Patch-Seq?

Should we be using housekeeping genes to normalize data?

Can we use NormFinder to find good house keeping genes?

[https://moma.dk/normfinder-software](https://moma.dk/normfinder-software)

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**Author:** ![jeremyinseattle](https://yyz1.discourse-cdn.com/flex027/user_avatar/community.brain-map.org/jeremyinseattle/32/50_2.png) [@jeremyinseattle](https://community.brain-map.org/u/jeremyinseattle)\
**Post date:** [July 18, 2022, 8:28pm UTC](https://community.brain-map.org/t/questions-on-house-keeping-genes/1726/2 "2022-07-18T20:28:41Z")

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We don’t typically use housekeeping genes for normalization of single cell RNA-seq data (Patch-Seq or otherwise). Please see [this response](https://community.brain-map.org/t/question-on-patch-seq-data/1724) about Patch-seq normalization, and a more general post about data normalization [here](https://community.brain-map.org/t/transcriptomics-rna-seq-microarray-data-normalization-faq/182). All that said, I am unfamiliar with “NormFinder” and can’t comment on its general utility.