# Showcase Symposium 2020 Project Talk Q&A: Transcriptomics Pipeline and Discoveries
**URL:** https://community.brain-map.org/t/showcase-symposium-2020-project-talk-q-a-transcriptomics-pipeline-and-discoveries/827
**Category:** Show & Tell
**Tags:** transcriptomics, showcase2020
**Created:** [December 12, 2020, 12:41am UTC](https://community.brain-map.org/t/showcase-symposium-2020-project-talk-q-a-transcriptomics-pipeline-and-discoveries/827 "2020-12-12T00:41:46Z")
**Posts on this page:** 5
**Page:** 1
### Author:  [@kaitlync](https://community.brain-map.org/u/kaitlync)
#### Post date: [December 12, 2020, 12:41am UTC](https://community.brain-map.org/t/showcase-symposium-2020-project-talk-q-a-transcriptomics-pipeline-and-discoveries/827/1 "2020-12-12T00:41:46Z")
At our virtual [Showcase Symposium 2020](https://alleninstitute.org/what-we-do/brain-science/events-training/showcase-symposium-2020/), Allen Institute for Brain Science teams present project talks highlighting their work. Please use this forum thread to ask questions for the speakers of the **Transcriptomics Pipeline and Discoveries** talk with Zizhen Yao, Cindy Van Velthoven, Jacqueline Bendrick, Elyse Morin, Amanda Mitchell, Qingzhong Ren, Yilin Zhao, and Michael Kuns. Please see the full list of [Showcase 2020 forum threads](https://community.brain-map.org/tag/showcase2020) to ask questions about other talks.
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### Author:  [@Anatoly](https://community.brain-map.org/u/Anatoly)
#### Post date: [December 15, 2020, 7:20pm UTC](https://community.brain-map.org/t/showcase-symposium-2020-project-talk-q-a-transcriptomics-pipeline-and-discoveries/827/2 "2020-12-15T19:20:07Z")
What are the technical limitations of manipulating the dataset sizes of the whole brain size?
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### Author:  [@a4willia](https://community.brain-map.org/u/a4willia)
#### Post date: [December 15, 2020, 7:23pm UTC](https://community.brain-map.org/t/showcase-symposium-2020-project-talk-q-a-transcriptomics-pipeline-and-discoveries/827/3 "2020-12-15T19:23:02Z")
Great talk, very exciting work. Any plans to expand the RNA-seq database to further into glia?
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### Author:  [@bosiljkatasic](https://community.brain-map.org/u/bosiljkatasic)
#### Post date: [December 15, 2020, 8:06pm UTC](https://community.brain-map.org/t/showcase-symposium-2020-project-talk-q-a-transcriptomics-pipeline-and-discoveries/827/4 "2020-12-15T20:06:53Z")
All our data will include non-neuronal cells, we just haven’t prioritized the analysis of those. But it is coming!
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### Author:  [@bosiljkatasic](https://community.brain-map.org/u/bosiljkatasic)
#### Post date: [December 15, 2020, 8:08pm UTC](https://community.brain-map.org/t/showcase-symposium-2020-project-talk-q-a-transcriptomics-pipeline-and-discoveries/827/5 "2020-12-15T20:08:46Z")
The key issue still persists: how does one: 1. create the large matrix of millions of cells x 10s of thousands of genes, 2. how does one manipulate this large matrix without having to load it all into memory.