# \#tissue-process **URL:** https://community.brain-map.org/tag/tissue-process/59.md [Latest](https://community.brain-map.org/latest.md) · [Categories](https://community.brain-map.org/categories.md) · [Tags](https://community.brain-map.org/tags.md) --- ## [Freezing of isolated nuclei](https://community.brain-map.org/t/freezing-of-isolated-nuclei/1081)
**Author:** [@Martina17](https://community.brain-map.org/u/Martina17)\ **Replies:** 2\ **Last updated:** [May 11, 2026, 1:54pm UTC](https://community.brain-map.org/t/freezing-of-isolated-nuclei/1081 "2026-05-11T13:54:26Z")
Hi, I am isolating nuclei from snap frozen brain tissue (prefrontal cortex). The nuclei are then used to perform single-nuclei RNA seq using 10x genomics technology. I normally extract the nuclei from the tissue and th… --- ## [DTT in nuclei isolation](https://community.brain-map.org/t/dtt-in-nuclei-isolation/1028)
**Author:** [@Martina17](https://community.brain-map.org/u/Martina17)\ **Replies:** 1\ **Last updated:** [April 13, 2021, 9:24pm UTC](https://community.brain-map.org/t/dtt-in-nuclei-isolation/1028 "2021-04-13T21:24:54Z")
Hi, I am working with isolation of nuclei from human prefrontal cortex. The protocol I am currently using can be summarized as follows: tissue homogenization using dounce homogenizer followed by a clean-up that is carr… --- ## [Recent complete normalized microarray datasets](https://community.brain-map.org/t/recent-complete-normalized-microarray-datasets/901)
**Author:** [@Eden](https://community.brain-map.org/u/Eden)\ **Replies:** 2\ **Last updated:** [January 27, 2021, 4:13am UTC](https://community.brain-map.org/t/recent-complete-normalized-microarray-datasets/901 "2021-01-27T04:13:41Z")
Hello, I was looking through the downloadable file provided in the Allen Brain Atlas (Microarray Data Download :: Allen Brain Atlas: Human Brain) , in the complete normalized microarray datasets, six brains are available… --- ## [Nuclei isolation for single-nuclei rna sequencing](https://community.brain-map.org/t/nuclei-isolation-for-single-nuclei-rna-sequencing/795)
**Author:** [@Martina17](https://community.brain-map.org/u/Martina17)\ **Replies:** 2\ **Last updated:** [January 27, 2021, 4:04am UTC](https://community.brain-map.org/t/nuclei-isolation-for-single-nuclei-rna-sequencing/795 "2021-01-27T04:04:21Z")
I am a research technician working in the field of neurology and I am currently trying to isolate nuclei from frozen human brain samples (prefrontal cortex). I would like to use these nuclei for single-nuclei RNA seq. T… --- ## [IRB approval statement?](https://community.brain-map.org/t/irb-approval-statement/796)
**Author:** [@sharastough](https://community.brain-map.org/u/sharastough)\ **Replies:** 2\ **Last updated:** [November 24, 2020, 7:09pm UTC](https://community.brain-map.org/t/irb-approval-statement/796 "2020-11-24T19:09:25Z")
Hello all, My undergraduate students are working with data from a number of data sets to get some research experience under these COVID-induced physical distancing conditions. I found a statement in the Aging, Dementia… --- ## [Ephys cell type database and duration of slices being ex-vivo](https://community.brain-map.org/t/ephys-cell-type-database-and-duration-of-slices-being-ex-vivo/574)
**Author:** [@MichaelFeyerabend](https://community.brain-map.org/u/MichaelFeyerabend)\ **Replies:** 1\ **Last updated:** [June 15, 2020, 8:19pm UTC](https://community.brain-map.org/t/ephys-cell-type-database-and-duration-of-slices-being-ex-vivo/574 "2020-06-15T20:19:53Z")
Hello everyone, I would like to look into a possible relationship between time of the slices being ex-vivo and intrinsic features obtained from intracellular recordings. The only time information, I found useful in that… --- ## [How to solubilize membrane proteins of human postmortem brain tissue for western blot analysis?](https://community.brain-map.org/t/how-to-solubilize-membrane-proteins-of-human-postmortem-brain-tissue-for-western-blot-analysis/358)
**Author:** [@Cheryl](https://community.brain-map.org/u/Cheryl)\ **Replies:** 2\ **Last updated:** [November 23, 2019, 3:08pm UTC](https://community.brain-map.org/t/how-to-solubilize-membrane-proteins-of-human-postmortem-brain-tissue-for-western-blot-analysis/358 "2019-11-23T15:08:51Z")
Hello, I would greatly appreciate any advice on preparing tissue for western blot with human postmortem tissue. I can get bands for my proteins, but they are variable and it seems to be most variable at the upper molec… --- ## [Myelin removal step for Isolation of Nuclei from Adult Human Brain Tissue for 10x Genomics Platform](https://community.brain-map.org/t/myelin-removal-step-for-isolation-of-nuclei-from-adult-human-brain-tissue-for-10x-genomics-platform/339)
**Author:** [@Luke\_E](https://community.brain-map.org/u/Luke_E)\ **Replies:** 1\ **Last updated:** [November 8, 2019, 8:08pm UTC](https://community.brain-map.org/t/myelin-removal-step-for-isolation-of-nuclei-from-adult-human-brain-tissue-for-10x-genomics-platform/339 "2019-11-08T20:08:57Z")
The Allen Institute has published laboratory protocols on protocol.io to enable the scientific community to utilize the techniques that drive our science. We received a question about myelin removal as part of our “Isola…