Especially interested in hippocampus CA2.
Yet another area where meshes can be intensely helpful.
Has anyone actually meshed an entire neuron with all its parts?
Especially interested in hippocampus CA2.
Yet another area where meshes can be intensely helpful.
Has anyone actually meshed an entire neuron with all its parts?
The electron microscopy Layer2/3 dataset (a small dataset that is part of the MICrONS project) does have mitochondria segmentation. This is in mouse visual cortex, in cortical layer 2-3. However this may be a good testbed for analysis. For more on the Layer 2/3 dataset, see: https://www.microns-explorer.org/phase1
In particular, this has separate segmentation and meshing of: cellular cytoplasm, nuclei, and mitochondria; and segmentation without meshing of postsynaptic densities, a proxy of the synaptic cleft. All of which you can see visualized in this neuroglancer link: https://layer23.microns-explorer.org/
Applying mitochondria and other organelle segmentation to the MICrONS cubic millimeter and other EM datasets is ongoing work, and not something we have available at the moment. But be aware that all EM data in production are also in mouse.