Hello everybody, i want to use the following normalized microarray datasets ( Microarray Data Download :: Allen Brain Atlas: Human Brain ) to perform a differential gene analysis on specific anatomical region of the brain. Therefore i use Bioconductor’s limma package and develop an R Script. Can you…

Dear jeremyinseattle, thanks for your answer! Your information was very helpful. I think it owuld be good to have it in white paper or in the Readme.txt file. I still try to find the best way to perform the Differential Gene Expression (DGE) analysis with the MicroarrrayExpression data file and ha…

Hi @waltheja . I don’t know the answer to 1 or 2 but will ask around and get back to you. For #3, I actually wrote a paper on that topic and have and R function to do the calculation for you, whichever method you try. In short, taking the highest expressed probe is the easiest way to do it and wil…

Normalized Microarray Datasets

Technical

jeremyinseattle October 10, 2022, 2:58pm 2

The short answer is yes, the data is log-transformed. A lot more information about data normalization in this (and other) atlases is in this post, if you are interested.

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Normalized Microarray Datasets

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