Reference Data - Mouse Connectivity

REFERENCE DATA

From this tab you can browse from a list of 5 reference dataset experiments. This datasets are also available to add to the cart so you can view them simultaneously with the Projection, BDA vs. rAAV and Transgenic datasets. To select a dataset, click the checkbox and then click “View Selections”. These selections will be stored as a “cookie” in your browser until you click the “Clear Selections” button.

ach reference dataset outlines the stain, the target of the stain as well as the staining pattern. Selecting a reference dataset will return the experiment ID, Mouse Strain, Sex, and Age.

Viewing the Experiments

Clicking on the experiment ID will launch a contact sheet viewer. Double-click an individual image in the contact sheet viewer to launch a High Resolution Image Viewer. The experiment ID, stains and section number are listed on the title banner of the viewer. Navigate through the images via the navigation panel in the main image viewer, or use the Keyboard Commands. To download the image, click the Download icon.

To adjust the image filters, click on the icon in the top right-hand corner of the viewer. The stains used in each channel are listed on the image filter adjustment tool. To turn off a channel, unclick the checkbox next to that channel. Moving the slider bars to the left will decrease the brightness of that channel, while moving the slider bars to the right will increase the channel brightness. Click the reset button to set to default levels.

Ref_data_color_guide

Keyboard Commands

In the High Resolution Image Viewer, use the keyboard commands to navigate through the image series. Keyboard commands include:

Key Function
F Advance to the next image in the series
D Go back to the previous image in the series
R Advance to the last image in the series
E Go back to the first image in the series
A Zoom in
Z Zoom out.
+ Zoom in. Please note that some keyboards may require the [Shift] key be held down while pressing the [+] key
- Zoom out

You can also use the arrow keys to pan the current image.