Hi @Cheryl,
Thanks for posting! The research we do at the Allen Institute for Brain Science has not leveraged Western blotting in protocols using human brain tissue, so we don’t have any specific advice to offer on this, but hopefully someone from the community might be able to weigh in.
We have encountered a few protocols in the literature that might be useful to refer to, as a starting point, though we have not tested any of them ourselves.
- Springer Protocols: Reis-de-Oliveira, et al, “A Complete Proteomic Workflow to Study Brain-Related Disorders via Postmortem Tissue.”
- Segerstrom, et al, “Minimizing Postsampling Degradation of Peptides by a Thermal Benchtop Tissue Stabilization Method.”
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Ferrer, et al, “Brain Protein Preservation Largely Depends on the Postmortem
Storage Temperature” - Blair, et al., “Individual Case Analysis of Postmortem Interval Time on Brain Tissue Preservation.”
Good luck with your experiments!