I am isolating nuclei from snap frozen brain tissue (prefrontal cortex). The nuclei are then used to perform single-nuclei RNA seq using 10x genomics technology.
I normally extract the nuclei from the tissue and then proceed to GEM generation and cDNA synthesys in the same day, which is quite a long procedure.
I was wondering if anybody is freezeing the nuclei suspension before proceeding to the 10x application. If so, which freezing medium do you use (i know of BAM Banker medium for example)? do you loose a lot of nuclei in the freezing - thawing process?
Thank you in advance