The Allen Institute has published laboratory protocols on protocol.io to enable the scientific community to utilize the techniques that drive our science. We received a question about myelin removal as part of our “Isolation of Nuclei from Adult Human Brain Tissue for 10x Genomics Platform” standard operating procedure: “…We have problems with the myelin removal step. We have used the removal beads from MACS and a magnetic stand from cell signaling. However, the beads won’t bind. Did you also experience this? And did you add some dynabeads too?”
We suspect others may have this same question so we plan to address it here, on our community forum, in the near future. Please stay tuned.