From your protocl - Isolation of Nuclei from Adult Human Brain Tissue for 10x Genomics Platform
It mentions that if the tissue is larger than 100mg, 7ml grinder is used. What is the upper limit for it? I have tissue blocks weight between 0.8g to 1g. Will 7ml grinder be sufficient, if not, can you recommend me a size, please?
And apart from the volume of homogenization buffer used differing between the small tissue and large tissue, does everything else stay the same? And if I need a bigger douncer (when 7ml grinder is not sufficient), will it be applicable for me to follow the protocol as it is and just change the homogenisation buffer at the beginning?