Is there documentation for neuropil trace calculation? Visual Coding 2P

Hi everyone,

I’m working with the Visual Coding 2P data, and I’m interested in understanding what methods and calculations were used to derive the provided neuropil traces. So far, I haven’t been able to find any details in the technical whitepapers or in other documentation online. If anyone could point me towards relevant resources or share insights on this process, I would greatly appreciate it.

Thank you!

This is documented in the Visual Coding Overview white paper that can be found here (Documentation: Brain Observatory) as well as in the Methods section of our platform paper found here: A large-scale standardized physiological survey reveals functional organization of the mouse visual cortex | Nature Neuroscience

Good luck!

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Thank you so much! Just to be absolutely certain: the neuropil corrected traces are obtained by get_corrected_fluorescence, and neuropil is obtained by get_neuropil?