RDS file for the seattle Alzheimer’s Disease Brain Cell Atlas

Hello, I wanted to annotate my single cell RNAseq umap with the seattle Alzheimer’s Disease Brain Cell Atlas, as well as do deconvolution of some spatial data. Is it possible to get the .rds seurat object that belongs to this atlas

Hi @bmk27e. The RDS Seurat objects for almost all of the data in MTG and DLPFC is available on cellxgene (here). L2/3 IT (and the whole taxonomy) have too many cells to be readable by R and therefore are only stored in h5ad format. It’s worth noting that the metadata is incomplete on cellxgene (we only shared fields to be visualized in the web tool)–for the complete set of data (in h5ad format) and metadata, please see our AWS site (here).