I am currently wondering why the summation of the DAPI detected cells via stardist in the ventricular systems (1157), fiber tracts (13868), and basic cell groups and regions (160543) objects in the attached images does not equal the number of DAPI detected cells in the root parent (176182) object. This phenomenon occurs for some other parent objects (lateral ventricle) as well in this particular example. I am wondering if this is simply due to ABBA’s hierarchy not including some areas such as some ventricular space as child objects or if there is a different issue. My goal is to create histograms for regions of interest but this is posing an issue since I do not know the exact cause. If anyone has ideas as to why this may be happening, please let me know.