Hello, allen institute.
I’m writing to inquire a question about the raw expression value of targeted gene that was indicated in ISH data of Allen Brain Atlas : mouse brain. I know the value of structure A is expression energy, but I can’t understand it. I wonder how raw expression value was caculated deeply.
In fact, I want to convert raw expression value to TPM. If you know how to do that, please teach me with detailed example.
I will looking for your team’s response.