I have a quick question about the Neuropixels dataset. I was thinking of analyzing some layer-specific things in the Neuropixels data (especially in layer 6b). The units are only labelled with a cortical region, but no layer - in order to assign them a layer I’ve used the ccfv3 coordinates of the units where available and the annotation matrix with reference space key ‘annotation/ccf_2017’.
This works fine and is very easy, however I’ve noticed that some units then end up in another (adjacent) cortical area than they were originally prescribed to (i.e. their original ‘ecephys_structure_acronym’). This just made me wonder: how trustworthy is this method? Has anyone tried this before (I haven’t found anything that similar in the forum…)? Is it possible to extract the layer information of these units?
I’m happy to post any code.
Thank you for any help!!