There is an option to download the gene grid expression data at 200 um resolution. Is it possible to download the grid expression data at a higher resolution say 25 um
if not is it possible to download the ish image series for each gene and register it to a reference atlas at 25um resolution to create a higher density map
We only have the 200 um resolution available.
In terms of creating a higher density map at 25 um, it’s technically possible but there are several steps. Two of note:
- The data were sampled at a 200 um resolution, so you’ll need an interpolation method to fill in the 25 um resolution.
- You’ll want to start by downloading our ISH and expression data from AllenSDK. You’ll need to write some software to assign the data into a grid. We have a software module that does this for connectivity data; it’s not directly applicable to this case, but conceptually similar.