Correspondence of anatomical divisions in MERFISH and scRNA-seq data

Thanks for these amazing datasets and tools! I have a question about the relationship between the anatomical division annotations in the spatial transcriptomics (MERFISH) and scRNA-seq data. I was looking at the gene Dbh, which is highly expressed in locus coeruleus neurons. In the MERFISH data these cells are annotated as P-unassigned (Pons), but in the scRNA-seq they are annotated as Medulla (MY). Do the two anatomical annotations match each other?

Thanks!

https://knowledge.brain-map.org/abcatlas#AQMBWkkzUlIwRlhMM0hZWEdWRTJTNQACRllZNE9NMkxVNUowWU9GMDdEUgADAQEyTlFUSUU3VEFNUDhQUUFITzRQAAIAAAQBAAKCmppchEbcXwODUEjZhHJ%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%2BAAAABAAFgCKzZIISHYcGsK0CBwIjRkZGRkZGAAMABkdFTkUABwAIAgAACEc0STRHRkpYSkI5QVRaM1BUWDEACUxWREJKQVc4Qkk1WVNTMVFVQkcACgALAW5vbmUAAm5vbmUAAwEEAQACIzAwMDAwMAADyAEABQEBAiMwMDAwMDAAA8gBAAAAAgQA

Hi,

The anatomical divisions are derived independently for spatial and RNAseq. RNAseq is based on region specific dissection and spatial TX data is based on image registration of the data. Both have their own levels of imprecision and especially in regions like the hindbrain it’s not very easy so discrepancies can arise.