How rto download single cell RNAseq data in patchseq

How To
1

Hi Allen Community,

I am trying to learn patch-seq data on your website, and I want to download patch-seq data for analysis. But I find the single-cell RNAseq data you put on NEMO is under control. How can I get access to obtain single-cell RNAseq data of patch-seq?

I know maybe I have to do some paperwork, may you let me know how to send my application

Thank you so much,

heather

2

Hi Heather, can you direct me to where are you are trying to download the data from? I need to know what source/link you are accessing before I can answer.

3

That is my fault. I want to download patch-seq data from your website on there url for data. I found that your patch-seq data are stored separately, so I click the red box on this picture to download RNAseq data

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I turn to the NEMO website, and I could not find the Source Data URL like the picture below

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There is the URL for data stored in NEMO RNAseq_data_stored_in_NEMO_url

Thanks

heather

4

Thanks Heather. On those links there should be a link /explanation on how to complete our NDA to gain access to our data. We are reaching out to our NeMO folks to restore this and I will contact you as soon as it is complete.

5

Thank you so much, Brian Lee, for helping me with the download link! Your assistance is greatly appreciated!

6

Hi Heather, here is the link to complete the NDA - this is required to access our data. This should be on the page you accessed and NeMO is currently working to make it more accessible. NeMO Archive - Accessing Controlled Access Data

7

Hi, Lee.

Thanks for your help! I really appreciate it. But now, it says my account doesn’t affiliate with the NIH-Recognized Research Institution. Is there any way I can do to deal with it? How can my account get permission from the institution? Should I sign a new account for it? If not, can I download these data from other websites?

Sincerely,
Heather

8

Hi Heather, accounts must be set up using an institutional email address.

9

Looks to me like the dataset link is now available at the NEMO landing page (NeMO Data Archive Assets), with a direct link to Index of /other/AIBS/AIBS_patchseq/transcriptome/scell/SMARTseq. This shouldn’t be controlled access data, @brian_lee maybe you were mixing this up with the human cell data?