Patch-seq Method
The method in which the morphology, electrophysiology and transcriptome (gene expression) profiles are assayed simultaneously from the same neuron. Here we detail the various steps and tools to generate data across species and brain regions.
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Slice Preparation and Blockface Imaging
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SMARTerSC Amplification for single-cell or single-nuclei RNASeq
Imaging
Morphology Reconstruction
Questions?
Refer to our Patch-seq methods paper: Scaled, high fidelity electrophysiological, morphological, and transcriptomic cell characterization | eLife
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analysis, celltype, electrophysiology, experiment-design, histology, how-to, single-cell-morphology, transcriptomics