Missing R2 FASTQ files for Patch-seq dataset

Technical
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1

Hello,

The methods section in the paper “Connecting single-cell transcriptomes to projectomes in mouse visual cortex" states that libraries were sequenced as “fifty-base-pair paired-end reads.” However, when downloading the raw FASTQ files from the NeMO archive (link), I can only find R1 files — no R2 files appear to be available for these samples.

I wanted to check on a couple of possibilities:

  1. Were the R2 files inadvertently omitted from the repository upload?

  2. Or was this particular batch actually sequenced as single-end, with the methods text referring to the general protocol rather than this specific dataset?

Thanks so much in advance.