Finding prevalence of transcripts in specific regions

Hi All,

I’m interested in finding the prevalence of RNA transcripts in the ABC atlas datasets, and using that to compare to some regional expression data of a certain protein.

I’ve been looking into using the MERFISH datasets from zhuang. In the paper, they mention that regions are annotated, but it doesn’t seem like that data is accessible - I’ve been trying to work back from the xyz coordinates included in the dataset, but that seems to be inaccurate (~10-15% are outside of atlas).

Has anyone tried to do this before, or have any insights?