Hello everyone, I am trying to map my entire mouse brain MERFISH sections of P4 & P7 to the Allen Brain Atlas and annotate the brain regions. Could anyone clarify the exact input data required for this process?

As of right now, we use some code internal to the Allen Institute for aligning adult MERFISH data to the common coordinate framework and assigning brain regions. A method for training a classifier to assign brain regions directly to cells is being developed, but isn’t ready at this time.

In addition to the post above, this post and this list of community resources may be of some value.